6AEZ
Crystal structure of human CCL5 trimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-08-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 56.556, 56.556, 154.103 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.090 - 1.630 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.22844 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EQT monomer |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.480 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.090 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.067 | 0.889 |
| Number of reflections | 30546 | |
| <I/σ(I)> | 48.73 | |
| Completeness [%] | 94.9 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 283 | 0.2M ammonium sulfate, 0.1M BIS-TRIS pH 5, 16% (w/v) polyethylene glycol 3350 |
