6ACI
Crystal structure of EPEC effector NleB in complex with FADD death domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2013-11-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97899 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.590, 84.590, 145.881 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.589 - 1.870 |
R-factor | 0.1666 |
Rwork | 0.164 |
R-free | 0.20940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e66 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.603 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.630 | 1.970 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmerge | 0.091 | 0.473 |
Number of reflections | 50430 | 7314 |
<I/σ(I)> | 16.3 | 5.5 |
Completeness [%] | 99.6 | 100 |
Redundancy | 10.4 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 4.5 M Sodium chloride, 100 mM HEPES pH 7.5 |