6A8R

Crystal structure of DUX4 HD2 domain associated with ERG DNA binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.9785
Spacegroup name	P 31
Unit cell lengths	32.550, 32.550, 126.591
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.189 - 1.600
R-factor	0.2008
Rwork	0.201
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5z2s 5z2t
RMSD bond length	0.013
RMSD bond angle	1.227
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.300	1.690
High resolution limit [Å]	1.600	1.600
Number of reflections	19696	2803
<I/σ(I)>	11.4
Completeness [%]	99.2
Redundancy	8.2
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 1000