6A88
Crystal Structure of T. gondii prolyl tRNA synthetase with Febrifugine and ATP Analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2017-11-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.910, 90.882, 93.028 |
| Unit cell angles | 89.91, 80.30, 75.58 |
Refinement procedure
| Resolution | 39.940 - 2.596 |
| R-factor | 0.1746 |
| Rwork | 0.172 |
| R-free | 0.21980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xif |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER (2.8.0) |
| Refinement software | PHENIX (1.15rc2_3428) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.830 | 2.670 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 73015 | 5337 |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 98.8 | 98.1 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.987 | 0.675 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 300 | Published |
