6A5S
Structure of 14-3-3 gamma in complex with TFEB 14-3-3 binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.088, 82.229, 88.092 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 44.044 - 2.099 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uzd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.875 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.099 | 2.099 |
| Rmerge | 0.142 | 0.664 |
| Number of reflections | 73457 | 3618 |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.3 | 289 | 0.1 M Tris-HCl, 0.2 M calcium acetate, and 20% (w/v) PEG 3350 |
