6ZX9

Crystal structure of SIV Vpr,fused to T4 lysozyme, isolated from moustached monkey, bound to human DDB1 and human DCAF1 (amino acid residues 1046-1396)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	265.898, 95.540, 98.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.066 - 2.520
R-factor	0.218135520807
Rwork	0.216
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zue 1qt6
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	XDS (XDSAPP)
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.070	2.560
High resolution limit [Å]	2.510	2.520
Number of reflections	85560	6264
<I/σ(I)>	9.78
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	0.996	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	8-10% PEG 4000 (w/v), 200 mM MgCl2, 100 mM HEPES-NaOH, pH 7.0-8.2.