6ZWK

Crystal structure of the phosphorylated C-terminal tail of histone H2AX in complex with a specific nanobody (C6 gammaXbody)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	87.412, 87.412, 105.409
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.490 - 1.550
R-factor	0.1508
Rwork	0.149
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz0
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (dev_3915)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.490	61.490	1.580
High resolution limit [Å]	1.550	8.490	1.550
Rmerge	0.091	0.042	2.268
Rmeas	0.095	0.045	2.384
Rpim	0.029	0.014	0.732
Total number of observations	1376764	7784	67802
Number of reflections	130679	795	6425
<I/σ(I)>	12.2	37.1	1
Completeness [%]	99.9	99.7	99.9
Redundancy	10.5	9.8	10.6
CC(1/2)	0.999	0.999	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% PEG 3350, 0.2M Sodium acetate