6ZVO
Crystal structure of unliganded MLKL executioner domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.99988 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.057, 53.501, 70.912 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 70.160 - 1.371 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4btf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.940 |
| Data reduction software | AutoProcess (1.1.7) |
| Data scaling software | AutoProcess |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.158 | 1.483 |
| High resolution limit [Å] | 1.375 | 1.375 |
| Number of reflections | 65990 | 3300 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 94.4 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.999 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 31 % Polyethylene glycol monomethyl ether 2,000, 0.15 M Potassium bromide, 0.1 M TRIS, pH 9 |
