6ZT0

Crystal structure of the Eiger TNF domain/Grindelwald extracellular domain complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.87313
Spacegroup name	P 3 2 1
Unit cell lengths	78.707, 78.707, 57.657
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.020 - 2.020
R-factor	0.1925
Rwork	0.191
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rj8
RMSD bond length	0.003
RMSD bond angle	0.582
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.660	2.050
High resolution limit [Å]	2.020	2.020
Rmerge	0.207	0.888
Number of reflections	13860	663
<I/σ(I)>	8.6	2.2
Completeness [%]	99.9
Redundancy	11.7
CC(1/2)	0.997	0.569

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M Tris-HCl pH 8.5 14 % PEG 4000 0.2 M MgCl2 concentration 28 mg/ml