6ZOF
Fusidic acid binding to the TM7/TM8 groove of AcrB-F380A T protomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 145.100, 162.327, 244.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.650 - 3.300 |
| R-factor | 0.2368 |
| Rwork | 0.236 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jmn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.166 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.650 | 49.650 | 3.360 |
| High resolution limit [Å] | 3.300 | 17.770 | 3.300 |
| Rmerge | 0.419 | 0.079 | 1.955 |
| Rmeas | 0.453 | 0.089 | 2.112 |
| Rpim | 0.170 | 0.039 | 0.793 |
| Total number of observations | 611897 | ||
| Number of reflections | 87562 | 627 | 4410 |
| <I/σ(I)> | 5.4 | 1.3 | |
| Completeness [%] | 100.0 | 95.8 | 100 |
| Redundancy | 7 | 6.1 | 7 |
| CC(1/2) | 0.972 | 0.979 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 291 | 0.05M ADA, PH 6.9, 0.15-0.25M AMMONIUM SULFATE, 5% GLYCEROL, 8-9% PEG4000 |
