6ZHS

Uba1 bound to two E2 (Ubc13) molecules

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9762
Spacegroup name	C 1 2 1
Unit cell lengths	177.710, 72.900, 139.520
Unit cell angles	90.00, 112.43, 90.00

Refinement procedure

Resolution	24.920 - 2.350
R-factor	0.2188
Rwork	0.216
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cmm
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.435
High resolution limit [Å]	2.350	2.350
Number of reflections	67708	398
<I/σ(I)>	6.97
Completeness [%]	66.1
Redundancy	5.6
CC(1/2)	0.987	0.424

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		273	ammonium sulfate, HEPES, PEG 3350