6ZHS
Uba1 bound to two E2 (Ubc13) molecules
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004839 | molecular_function | ubiquitin activating enzyme activity |
A | 0005515 | molecular_function | protein binding |
A | 0005524 | molecular_function | ATP binding |
A | 0005634 | cellular_component | nucleus |
A | 0005737 | cellular_component | cytoplasm |
A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
A | 0006974 | biological_process | DNA damage response |
A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
A | 0016567 | biological_process | protein ubiquitination |
A | 0016874 | molecular_function | ligase activity |
A | 0036211 | biological_process | protein modification process |
B | 0000209 | biological_process | protein polyubiquitination |
B | 0000329 | cellular_component | fungal-type vacuole membrane |
B | 0004842 | molecular_function | ubiquitin-protein transferase activity |
B | 0005515 | molecular_function | protein binding |
B | 0005524 | molecular_function | ATP binding |
B | 0005634 | cellular_component | nucleus |
B | 0005737 | cellular_component | cytoplasm |
B | 0005829 | cellular_component | cytosol |
B | 0006301 | biological_process | postreplication repair |
B | 0010994 | biological_process | free ubiquitin chain polymerization |
B | 0016567 | biological_process | protein ubiquitination |
B | 0016740 | molecular_function | transferase activity |
B | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
B | 0044395 | biological_process | protein targeting to vacuolar membrane |
B | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
B | 0070534 | biological_process | protein K63-linked ubiquitination |
C | 0000209 | biological_process | protein polyubiquitination |
C | 0000329 | cellular_component | fungal-type vacuole membrane |
C | 0004842 | molecular_function | ubiquitin-protein transferase activity |
C | 0005515 | molecular_function | protein binding |
C | 0005524 | molecular_function | ATP binding |
C | 0005634 | cellular_component | nucleus |
C | 0005737 | cellular_component | cytoplasm |
C | 0005829 | cellular_component | cytosol |
C | 0006301 | biological_process | postreplication repair |
C | 0010994 | biological_process | free ubiquitin chain polymerization |
C | 0016567 | biological_process | protein ubiquitination |
C | 0016740 | molecular_function | transferase activity |
C | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
C | 0044395 | biological_process | protein targeting to vacuolar membrane |
C | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
C | 0070534 | biological_process | protein K63-linked ubiquitination |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 9 |
Details | binding site for residue SO4 A 1101 |
Chain | Residue |
A | GLY443 |
A | ALA444 |
A | ILE445 |
A | ARG481 |
A | GLN482 |
A | ASP544 |
A | HOH1205 |
A | HOH1244 |
A | HOH1245 |
site_id | AC2 |
Number of Residues | 7 |
Details | binding site for residue SO4 A 1102 |
Chain | Residue |
A | ARG21 |
A | ASN478 |
A | ARG481 |
A | ASP544 |
A | HOH1214 |
A | HOH1259 |
A | HOH1263 |
site_id | AC3 |
Number of Residues | 6 |
Details | binding site for residue GOL A 1103 |
Chain | Residue |
A | LEU26 |
A | GLY27 |
A | LYS28 |
A | GLU29 |
A | ALA30 |
A | HOH1219 |
site_id | AC4 |
Number of Residues | 4 |
Details | binding site for residue GOL A 1104 |
Chain | Residue |
A | VAL467 |
A | ASN514 |
A | HOH1212 |
A | HOH1322 |
site_id | AC5 |
Number of Residues | 8 |
Details | binding site for residue GOL A 1105 |
Chain | Residue |
A | ASP282 |
A | LYS285 |
A | PHE391 |
A | ASP392 |
A | SER393 |
A | GLU395 |
A | GLU880 |
A | LYS895 |
site_id | AC6 |
Number of Residues | 7 |
Details | binding site for residue GOL A 1106 |
Chain | Residue |
A | GLU215 |
A | ILE254 |
A | ASN607 |
A | ARG713 |
A | PHE846 |
A | ILE847 |
A | GLU848 |
site_id | AC7 |
Number of Residues | 6 |
Details | binding site for residue GOL A 1107 |
Chain | Residue |
A | LYS33 |
A | THR172 |
A | ALA377 |
A | CYS378 |
A | SER379 |
A | GLY380 |
site_id | AC8 |
Number of Residues | 7 |
Details | binding site for residue GOL A 1108 |
Chain | Residue |
A | SER17 |
A | GLU475 |
A | LYS476 |
A | SER477 |
A | ASN478 |
A | ASP824 |
A | HOH1267 |
Functional Information from PROSITE/UniProt
site_id | PS00183 |
Number of Residues | 16 |
Details | UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNIdrl.GrICLdvL |
Chain | Residue | Details |
B | TYR76-LEU91 |
site_id | PS00536 |
Number of Residues | 9 |
Details | UBIQUITIN_ACTIVAT_1 Ubiquitin-activating enzyme signature 1. KACSGKFtP |
Chain | Residue | Details |
A | LYS376-PRO384 |
site_id | PS00865 |
Number of Residues | 9 |
Details | UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PLCTLRsFP |
Chain | Residue | Details |
A | PRO598-PRO606 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 2 |
Details | ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388 |
Chain | Residue | Details |
B | CYS87 | |
C | CYS87 |
site_id | SWS_FT_FI2 |
Number of Residues | 4 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
Chain | Residue | Details |
B | LYS92 | |
A | ASP470 | |
C | LYS92 | |
A | LYS494 | |
A | ASP544 |
site_id | SWS_FT_FI3 |
Number of Residues | 1 |
Details | MOD_RES: N-acetylserine => ECO:0007744|PubMed:22814378 |
Chain | Residue | Details |
A | SER2 |
site_id | SWS_FT_FI4 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17287358 |
Chain | Residue | Details |
A | SER265 |
site_id | SWS_FT_FI5 |
Number of Residues | 1 |
Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956 |
Chain | Residue | Details |
A | SER914 |
site_id | SWS_FT_FI6 |
Number of Residues | 3 |
Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
Chain | Residue | Details |
A | LYS595 | |
A | LYS608 |
Catalytic Information from CSA
site_id | MCSA1 |
Number of Residues | 8 |
Details | M-CSA 307 |
Chain | Residue | Details |
A | ARG21 | electrostatic stabiliser, hydrogen bond donor, steric role |
A | ARG481 | electrostatic stabiliser, hydrogen bond donor, steric role |
A | ASP544 | steric role |
A | CYS600 | activator, covalently attached, hydrogen bond donor, nucleophile, proton donor |
A | THR601 | hydrogen bond acceptor, hydrogen bond donor, increase acidity, increase nucleophilicity, proton acceptor, proton donor, proton relay |
A | ARG603 | electrostatic stabiliser, hydrogen bond donor |
A | ASN781 | electrostatic stabiliser, hydrogen bond donor |
A | ASP782 | electrostatic stabiliser, hydrogen bond donor |
site_id | MCSA2 |
Number of Residues | 5 |
Details | M-CSA 939 |
Chain | Residue | Details |
A | CYS600 | nucleofuge |
A | THR601 | modifies pKa |
A | ARG603 | electrostatic stabiliser |
A | ASN781 | electrostatic stabiliser |
A | ASP782 | electrostatic stabiliser |