6ZHS
Uba1 bound to two E2 (Ubc13) molecules
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004839 | molecular_function | ubiquitin activating enzyme activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0006511 | biological_process | ubiquitin-dependent protein catabolic process |
| A | 0006974 | biological_process | DNA damage response |
| A | 0008641 | molecular_function | ubiquitin-like modifier activating enzyme activity |
| A | 0016567 | biological_process | protein ubiquitination |
| A | 0016874 | molecular_function | ligase activity |
| A | 0036211 | biological_process | protein modification process |
| B | 0000209 | biological_process | protein polyubiquitination |
| B | 0000329 | cellular_component | fungal-type vacuole membrane |
| B | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006301 | biological_process | postreplication repair |
| B | 0010994 | biological_process | free ubiquitin chain polymerization |
| B | 0016567 | biological_process | protein ubiquitination |
| B | 0016740 | molecular_function | transferase activity |
| B | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
| B | 0044395 | biological_process | protein targeting to vacuolar membrane |
| B | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| B | 0070534 | biological_process | protein K63-linked ubiquitination |
| C | 0000209 | biological_process | protein polyubiquitination |
| C | 0000329 | cellular_component | fungal-type vacuole membrane |
| C | 0004842 | molecular_function | ubiquitin-protein transferase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006301 | biological_process | postreplication repair |
| C | 0010994 | biological_process | free ubiquitin chain polymerization |
| C | 0016567 | biological_process | protein ubiquitination |
| C | 0016740 | molecular_function | transferase activity |
| C | 0031371 | cellular_component | ubiquitin conjugating enzyme complex |
| C | 0044395 | biological_process | protein targeting to vacuolar membrane |
| C | 0061631 | molecular_function | ubiquitin conjugating enzyme activity |
| C | 0070534 | biological_process | protein K63-linked ubiquitination |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 9 |
| Details | binding site for residue SO4 A 1101 |
| Chain | Residue |
| A | GLY443 |
| A | ALA444 |
| A | ILE445 |
| A | ARG481 |
| A | GLN482 |
| A | ASP544 |
| A | HOH1205 |
| A | HOH1244 |
| A | HOH1245 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 A 1102 |
| Chain | Residue |
| A | ARG21 |
| A | ASN478 |
| A | ARG481 |
| A | ASP544 |
| A | HOH1214 |
| A | HOH1259 |
| A | HOH1263 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1103 |
| Chain | Residue |
| A | LEU26 |
| A | GLY27 |
| A | LYS28 |
| A | GLU29 |
| A | ALA30 |
| A | HOH1219 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 1104 |
| Chain | Residue |
| A | VAL467 |
| A | ASN514 |
| A | HOH1212 |
| A | HOH1322 |
| site_id | AC5 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 1105 |
| Chain | Residue |
| A | ASP282 |
| A | LYS285 |
| A | PHE391 |
| A | ASP392 |
| A | SER393 |
| A | GLU395 |
| A | GLU880 |
| A | LYS895 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 1106 |
| Chain | Residue |
| A | GLU215 |
| A | ILE254 |
| A | ASN607 |
| A | ARG713 |
| A | PHE846 |
| A | ILE847 |
| A | GLU848 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1107 |
| Chain | Residue |
| A | LYS33 |
| A | THR172 |
| A | ALA377 |
| A | CYS378 |
| A | SER379 |
| A | GLY380 |
| site_id | AC8 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 1108 |
| Chain | Residue |
| A | SER17 |
| A | GLU475 |
| A | LYS476 |
| A | SER477 |
| A | ASN478 |
| A | ASP824 |
| A | HOH1267 |
Functional Information from PROSITE/UniProt
| site_id | PS00183 |
| Number of Residues | 16 |
| Details | UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNIdrl.GrICLdvL |
| Chain | Residue | Details |
| B | TYR76-LEU91 |
| site_id | PS00536 |
| Number of Residues | 9 |
| Details | UBIQUITIN_ACTIVAT_1 Ubiquitin-activating enzyme signature 1. KACSGKFtP |
| Chain | Residue | Details |
| A | LYS376-PRO384 |
| site_id | PS00865 |
| Number of Residues | 9 |
| Details | UBIQUITIN_ACTIVAT_2 Ubiquitin-activating enzyme active site. PLCTLRsFP |
| Chain | Residue | Details |
| A | PRO598-PRO606 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Glycyl thioester intermediate => ECO:0000255|PROSITE-ProRule:PRU00388 |
| Chain | Residue | Details |
| B | CYS87 | |
| C | CYS87 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| B | LYS92 | |
| A | ASP470 | |
| C | LYS92 | |
| A | LYS494 | |
| A | ASP544 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | MOD_RES: N-acetylserine => ECO:0007744|PubMed:22814378 |
| Chain | Residue | Details |
| A | SER2 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:17287358 |
| Chain | Residue | Details |
| A | SER265 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18407956 |
| Chain | Residue | Details |
| A | SER914 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| A | LYS595 | |
| A | LYS608 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 307 |
| Chain | Residue | Details |
| A | ARG21 | electrostatic stabiliser, hydrogen bond donor, steric role |
| A | ARG481 | electrostatic stabiliser, hydrogen bond donor, steric role |
| A | ASP544 | steric role |
| A | CYS600 | activator, covalently attached, hydrogen bond donor, nucleophile, proton donor |
| A | THR601 | hydrogen bond acceptor, hydrogen bond donor, increase acidity, increase nucleophilicity, proton acceptor, proton donor, proton relay |
| A | ARG603 | electrostatic stabiliser, hydrogen bond donor |
| A | ASN781 | electrostatic stabiliser, hydrogen bond donor |
| A | ASP782 | electrostatic stabiliser, hydrogen bond donor |
| site_id | MCSA2 |
| Number of Residues | 5 |
| Details | M-CSA 939 |
| Chain | Residue | Details |
| A | CYS600 | nucleofuge |
| A | THR601 | modifies pKa |
| A | ARG603 | electrostatic stabiliser |
| A | ASN781 | electrostatic stabiliser |
| A | ASP782 | electrostatic stabiliser |
