6Z87
human GTP cyclohydrolase I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.999860 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 109.877, 109.877, 387.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.156 - 2.564 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fb1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.156 | 2.904 |
| High resolution limit [Å] | 2.564 | 2.564 |
| Number of reflections | 44678 | 21546 |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 93.6 | 77.2 |
| Redundancy | 18.6 | |
| CC(1/2) | 0.997 | 0.670 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 1.26 M Ammonium Sulfate, 0.1 M TRIS pH 8.5 and 0.2 M Lithium sulfate |
