6Z4A

Structure of the human SAS-6 N-terminal domain, F131E mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	59.590, 65.130, 38.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.980 - 1.460
R-factor	0.201
Rwork	0.199
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y3v
RMSD bond length	0.009
RMSD bond angle	1.100
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.980	1.490
High resolution limit [Å]	1.460	1.460
Rmerge	0.060	1.118
Rmeas	0.066	1.238
Rpim	0.027	0.526
Number of reflections	26380	1273
<I/σ(I)>	14.6	1.4
Completeness [%]	99.9	99.5
Redundancy	6.1	5.4
CC(1/2)	0.999	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M HEPES 30% w/v PEG 6000 Cryoprotected by addition of 20% v/v glycerol