6Z49

Crystal structure of deubiquitinase Mindy2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.07252
Spacegroup name	P 1 21 1
Unit cell lengths	67.115, 120.420, 78.378
Unit cell angles	90.00, 92.94, 90.00

Refinement procedure

Resolution	47.720 - 2.000
R-factor	0.2172
Rwork	0.215
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jkn
RMSD bond length	0.008
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.720	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.052	0.673
Rmeas	0.059	0.773
Rpim	0.028	0.376
Total number of observations	344672
Number of reflections	83541	4588
<I/σ(I)>	13.9
Completeness [%]	99.7	99.7
Redundancy	4.1	4.1
CC(1/2)	0.999	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	0.1 M Bis-Tris pH 6.0, 0.2 M Magnesium chloride and 25% PEG 3350