6Z3L

Repulsive Guidance Molecule C (RGMC, Hemojuvelin, HJV, HFE2) in complex with Growth Differentiation Factor 5 (GDF5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 62 2 2
Unit cell lengths	98.820, 98.820, 99.230
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	85.580 - 2.513
R-factor	0.2395
Rwork	0.238
R-free	0.26730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ui1
RMSD bond length	0.002
RMSD bond angle	0.386
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13rc2_2986: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.580	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.120
Rpim		1.125
Number of reflections	10324	721
<I/σ(I)>	23.9	0.7
Completeness [%]	99.0
Redundancy	47.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	1 M LiCl, 0.1 M citric acid pH 4.0, 8% gamma-butyrolactone.