6Z2P
Crystal structure of catalytic inactive OgpA from Akkermansia muciniphila in complex with an O-glycopeptide (glycodrosocin) substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979181 |
| Spacegroup name | I 4 |
| Unit cell lengths | 126.920, 126.920, 65.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.873 - 2.160 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6z2d |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.873 | 2.238 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.026 | 0.433 |
| Rmeas | 0.037 | 0.612 |
| Number of reflections | 27920 | 2619 |
| <I/σ(I)> | 14.42 | 1.39 |
| Completeness [%] | 99.0 | 93.63 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.992 | 0.433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM MIB pH 5.0 and 25% (w/v) PEG 1,500 |
