6Z10

Crystal structure of a humanized (K18E, K269N) rat succinate receptor SUCNR1 (GPR91) in complex with a nanobody and antagonist

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	C 1 2 1
Unit cell lengths	76.839, 151.003, 68.211
Unit cell angles	90.00, 112.29, 90.00

Refinement procedure

Resolution	63.120 - 2.269
R-factor	0.2048
Rwork	0.204
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rnk
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7 (19-MAR-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.120	63.115	2.444
High resolution limit [Å]	2.269	6.761	2.269
Rmerge	0.172	0.043	2.274
Rmeas	0.213	0.054	2.746
Rpim	0.124	0.032	1.523
Number of reflections	25647	1281	1283
<I/σ(I)>	5.6		0.6
Completeness [%]	90.6	99.5	19.5
Redundancy	5.4	5.2	6.2
CC(1/2)	0.995	0.999	0.214

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293.15	50 mM ADA pH 7.0, 28% (w/v) PEG MME 550, 0.55 M (NH4)2SO4, 200 microM RAW241, 2% (v/v) DMSO