6YPX

Human histidine triad nucleotide-binding protein 2 (hHINT2) refined to 2.11 A in C2221 space group

This is a non-PDB format compatible entry.

Replaces:  4NJZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9669
Spacegroup name	C 2 2 21
Unit cell lengths	138.411, 153.992, 74.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.940 - 2.110
Rwork	0.148
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw2
RMSD bond length	0.012
RMSD bond angle	1.613
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP (11.2.05)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	102.940	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.220	1.041
Rmeas	0.240	1.194
Rpim	0.094	0.568
Number of reflections	46164	3623
<I/σ(I)>	11.3	2
Completeness [%]	99.6	96.4
Redundancy	12.2	7.4
CC(1/2)	0.994	0.697

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	0.2 M sodium citrate, 0.1 M sodium cacodylate, 30 % 2-propanol