6YPC
Crystal structure of the kinetochore subunits H/I/K/T/W penta-complex from S. cerevisiae at 2.9 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9465 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 132.577, 132.577, 241.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.850 - 2.900 |
| R-factor | 0.2312 |
| Rwork | 0.228 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5Z08 and 3B0D |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.263 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.070 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.368 | |
| Rpim | 0.047 | 1.826 |
| Number of reflections | 24344 | 3860 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 100.0 | |
| Redundancy | 63.3 | |
| CC(1/2) | 0.999 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M NaH2PO4 and 0.38M K2HPO4 with protein at 5.5 mg/ml |
