6YLV

Translation initiation factor 4E in complex with 4-Cl-Bn7GpppG mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	38.350, 38.390, 148.080
Unit cell angles	85.28, 88.42, 77.23

Refinement procedure

Resolution	49.190 - 2.660
R-factor	0.203474516796
Rwork	0.201
R-free	0.25527
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.006
RMSD bond angle	0.747
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.755
High resolution limit [Å]	2.660	2.660
Rmerge	0.112	0.571
Rmeas	0.126	0.680
Rpim	0.056	0.361
Number of reflections	22090	1453
<I/σ(I)>	10.32	2.04
Completeness [%]	93.6	60.42
Redundancy	4.7	2.8
CC(1/2)	0.991	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 M Bicine pH 8.5, 0.2 M sodium formate, 19 % w/v PEGMME 5000