6YK5

Structure of the AMPA receptor GluA2o ligand-binding domain (S1S2J) in complex with the compound (S)-1-(2'-Amino-2'-carboxyethyl)-5,7-dihydrofuro[3,4-d]- pyrimidine-2,4(1H,3H)-dione at resolution 1.15A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-3
Synchrotron site	MAX II
Beamline	I911-3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	59.075, 96.331, 48.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.778 - 1.150
R-factor	0.1108
Rwork	0.110
R-free	0.13010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1syh
Data reduction software	XDS
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.212	26.778	1.210
High resolution limit [Å]	1.150	3.640	1.150
Rmerge		0.035	0.385
Rmeas	0.069	0.040	0.452
Rpim	0.032	0.019	0.231
Total number of observations	450981	15244	44337
Number of reflections	97655	3359	12982
<I/σ(I)>	14.2	35.7	3.2
Completeness [%]	98.6	99	90.7
Redundancy	4.6	4.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	279	PEG4000, lithium sulfate, acetate