6YIE

Structure of a Borealin-INCENP-Survivin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	110
Detector technology	PIXEL
Collection date	2017-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	P 21 21 21
Unit cell lengths	59.536, 78.824, 125.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.700 - 3.490
R-factor	0.2714
Rwork	0.269
R-free	0.32430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qfa
RMSD bond length	0.004
RMSD bond angle	0.810
Data reduction software	Aimless
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.820	3.830
High resolution limit [Å]	3.490	3.490
Rmerge	0.162	0.435
Number of reflections	6947	1644
<I/σ(I)>	3.5	1.7
Completeness [%]	88.5
Redundancy	3.7
CC(1/2)	0.992	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	298	5 % (w/v) PEG 3,350, 50 mM MES pH 6.0