6YE3
IL-2 in complex with a Fab fragment from UFKA-20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 238.384, 92.547, 121.892 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.890 |
| R-factor | 0.2185 |
| Rwork | 0.215 |
| R-free | 0.28330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b6f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.317 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.993 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Number of reflections | 114189 | 5708 |
| <I/σ(I)> | 5.74 | 0.38 |
| Completeness [%] | 99.7 | 98.21 |
| Redundancy | 6.9 | 6.6 |
| CC(1/2) | 0.992 | 0.186 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10.86% (v/v) PEG 8000, 5.76% (v/v) ethylene glycol, 100 mM HEPES, pH 7.48 |
