6YA7

Cdc7-Dbf4 bound to an Mcm2-S40 derived bivalent substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	62.280, 62.280, 234.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.200 - 1.670
R-factor	0.1577
Rwork	0.156
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5MH9
RMSD bond length	0.010
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3707)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.200	1.710
High resolution limit [Å]	1.670	1.670
Rmerge	0.098	0.724
Number of reflections	54941	5401
<I/σ(I)>	19.4
Completeness [%]	100.0
Redundancy	12.5
CC(1/2)	1.000	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.1M PCTP pH7, 18% PEG 1500, 8% MPD