6YA6

Minimal construct of Cdc7-Dbf4 bound to XL413

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 41 21 2
Unit cell lengths	61.640, 61.640, 233.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.850 - 1.440
R-factor	0.1679
Rwork	0.167
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5MH9
RMSD bond length	0.006
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (dev_3707)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.610	1.491
High resolution limit [Å]	1.440	1.440
Rmerge	0.049	0.584
Number of reflections	82613	7764
<I/σ(I)>	26.4
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	1.000	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291.15	0.1M PCTP pH7, 18% PEG 1500, 8% MPD