6Y6B

Crystal structure of human 14-3-3 gamma in complex with CaMKK2 14-3-3 binding motif Ser100 and 16-OMe-Fusicoccin H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-24
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 63 2 2
Unit cell lengths	226.266, 226.266, 72.993
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.750 - 3.080
R-factor	0.244
Rwork	0.242
R-free	0.28180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b05
RMSD bond length	0.001
RMSD bond angle	0.357
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	3.260
High resolution limit [Å]	3.080	3.080
Rmeas	0.280	4.970
Number of reflections	20904	3285
<I/σ(I)>	11.53	0.69
Completeness [%]	99.9	99.7
Redundancy	21.72	22
CC(1/2)	0.999	0.226

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	PEG400, HEPES, magnesium chloride, hexafluoropropanol