6Y4Y

The crystal structure of human MACROD2 in space group P41212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-27
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 41 21 2
Unit cell lengths	95.650, 95.650, 258.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.830 - 1.750
R-factor	0.2055
Rwork	0.204
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iqy
RMSD bond length	0.007
RMSD bond angle	1.439
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.830	47.830	1.800
High resolution limit [Å]	1.750	7.830	1.750
Rmerge	0.107	0.068	1.542
Rmeas	0.112	0.072	1.613
Total number of observations	1472885
Number of reflections	121564	1572	8864
<I/σ(I)>	13.27	32.26	1.7
Completeness [%]	100.0	99.4	100
Redundancy	12.116	10.402	11.75
CC(1/2)	0.998	0.998	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	0.2 M Ammonium tartarate dibasic pH 6.7, 20% PEG 3350