6Y4P

Calmodulin N53I variant bound to cardiac ryanodine receptor (RyR2) calmodulin binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-11
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	37.590, 42.360, 90.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.180 - 2.133
R-factor	0.188711047719
Rwork	0.182
R-free	0.24621
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bcx
RMSD bond length	0.008
RMSD bond angle	0.735
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.180	2.209
High resolution limit [Å]	2.133	2.133
Number of reflections	8489	831
<I/σ(I)>	9.45
Completeness [%]	99.8
Redundancy	5.9
CC(1/2)	0.993	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	0.1 M Sodium Acetate pH 4.70 and 23 % PEG 550 MME