6Y09
Crystal structure of Atg16L in complex with GTP-bound Rab33B (Q92L)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97714 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.950, 132.420, 155.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.168 - 2.400 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.23110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3Q8T and 1Z06 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.170 | 2.486 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 46731 | 4618 |
<I/σ(I)> | 17.05 | 2.67 |
Completeness [%] | 99.9 | 99.87 |
Redundancy | 12.9 | 13.6 |
CC(1/2) | 0.871 | 0.871 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277.15 | 14% PEG3350, 0.2 M Potassium thiocyanate and 0.1 M Sodium potassium phosphate |