6XUP

Archaeoglobus fulgidus Argonaute protein with DNA oligoduplex 5'-pATCGTGGCCACGAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97630
Spacegroup name	P 1
Unit cell lengths	51.798, 60.872, 101.718
Unit cell angles	76.56, 75.59, 79.39

Refinement procedure

Resolution	40.716 - 1.900
R-factor	0.1856
Rwork	0.181
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 2w42
Data reduction software	XDS
Data scaling software	pointless (1.10.26)
Phasing software	MOLREP (11.6.04)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.500	41.500	1.940
High resolution limit [Å]	1.900	9.870	1.900
Rmerge	0.053	0.030	0.955
Rmeas	0.062	0.036	1.106
Rpim	0.032	0.019	0.557
Number of reflections	91396	572	4556
<I/σ(I)>	11.2
Completeness [%]	91.6	87.9	91.2
Redundancy	3.8	3.5	3.9
CC(1/2)	0.999	0.997	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	280	Na-Cacodylate pH 5.5 0.05 M, KCl 0.12 M, MgCl2 0.01 M, PEG3350 7%, DTT_0.005 M, glycerol 5% (w/v)