6XLV

Crystal structure of leukemia-associated N196K mutant of U2AF65 bound to AdML splice site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	43.335, 63.048, 76.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.450 - 1.400
R-factor	0.143
Rwork	0.141
R-free	0.16860
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5ev3
RMSD bond length	0.010
RMSD bond angle	1.209
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	Aimless (0.7.4)
Phasing software	PHENIX (1.17.1)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.450	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.055	0.677
Rpim	0.024	0.381
Number of reflections	76426	1439
<I/σ(I)>	13.1	1.3
Completeness [%]	96.0	70.7
Redundancy	6.2	3.8
CC(1/2)	0.998	0.703

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PEG 3350, 3.4 M sodium malonate pH 7, sucrose, LDAO