6XKI

Crystal structure of eIF4A-I in complex with RNA bound to des-MePateA, a pateamine A analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	I 2 2 2
Unit cell lengths	66.785, 99.942, 153.663
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.580 - 2.870
R-factor	0.2238
Rwork	0.222
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5zc9
RMSD bond length	0.003
RMSD bond angle	0.470
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.870	2.870
Number of reflections	11588	453
<I/σ(I)>	12.25
Completeness [%]	96.6
Redundancy	11.4
CC(1/2)	0.984	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	298	0.1 M MES pH 6.7, 1.9 M Ammonium Sulfate