6X0P
Ash1L SET domain Q2265A mutant in complex with AS-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.131, 62.315, 72.969 |
| Unit cell angles | 87.76, 85.55, 90.04 |
Refinement procedure
| Resolution | 40.860 - 1.690 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ynm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.860 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.700 |
| Number of reflections | 101290 | 9261 |
| <I/σ(I)> | 14.4 | 2.1 |
| Completeness [%] | 95.8 | |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 290 | 0.1 M Tris pH 8.5, 0.2 M MgCl2, 30% PEG4000 |
