6WMS
Crystal Structure of Human REV-ERBbeta Ligand Binding Domain Co-Bound to Heme and NCoR ID2 Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.586, 73.400, 59.673 |
Unit cell angles | 90.00, 101.91, 90.00 |
Refinement procedure
Resolution | 43.699 - 2.000 |
R-factor | 0.1913 |
Rwork | 0.188 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cqv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.892 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.313 | |
Number of reflections | 31527 | 31527 |
<I/σ(I)> | 10.44 | 1.94 |
Completeness [%] | 99.1 | 98.93 |
Redundancy | 2 | |
CC(1/2) | 1.000 | 0.754 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Mg formate dihydrate, 20% w/v PEG 3350 |