6WCW

Structure of human Rubicon RH domain in complex with GTP-bound Rab7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.283
Spacegroup name	I 1 2 1
Unit cell lengths	60.040, 45.330, 186.277
Unit cell angles	90.00, 98.51, 90.00

Refinement procedure

Resolution	54.220 - 2.800
R-factor	0.2024
Rwork	0.200
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yhn
RMSD bond length	0.009
RMSD bond angle	1.054
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.110	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	12498	1234
<I/σ(I)>	6.4	2.2
Completeness [%]	99.1	98.8
Redundancy	2.8	2.8
CC(1/2)	0.986	0.715

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	6% (w/v) PEG 8000, 100mM Tris-HCl pH 7.4, 15mM TCEP