6W9A

RNF12 RING domain in complex with Ube2e2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-25
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 31
Unit cell lengths	81.355, 81.355, 98.247
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.296 - 2.300
R-factor	0.182
Rwork	0.180
R-free	0.21860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y6l
RMSD bond length	0.008
RMSD bond angle	0.959
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHASER
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.680	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.081	1.129
Rmeas	0.089	1.239
Rpim	0.037	0.510
Total number of observations	188008
Number of reflections	32329	3171
<I/σ(I)>	15.9
Completeness [%]	99.9	99.9
Redundancy	5.8	5.8
CC(1/2)	0.999	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	20% PEG 3350, 100 mM Na/K phosphate, 100 mM bis-Tris propane