6W8W

Crystal structure of mouse DNMT1 in complex with CCG DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	89.794, 152.593, 95.631
Unit cell angles	90.00, 94.74, 90.00

Refinement procedure

Resolution	48.440 - 3.000
R-factor	0.2398
Rwork	0.238
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4da4
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	2.990	6.460	3.000
Rmerge	0.274	0.087	1.034
Rmeas	0.302	0.094	1.162
Rpim	0.123	0.036	0.517
Number of reflections	50826	5192	4994
<I/σ(I)>	1.9
Completeness [%]	98.2	98.3	96.8
Redundancy	5.6	6.5	4.4
CC(1/2)		0.996	0.304

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	277	0.1 M Sodium Citrate, 10 mM ZnCl2