6W3I

Crystal structure of a FAM46C mutant in complex with Plk4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-09
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 31 2 1
Unit cell lengths	142.291, 142.291, 147.884
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.334 - 3.802
R-factor	0.2218
Rwork	0.217
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4n7z
RMSD bond length	0.004
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Rpim	0.029
Number of reflections	17441	866
<I/σ(I)>	27.8
Completeness [%]	100.0
Redundancy	17.8
CC(1/2)		0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	25% v/v pentaerythritol ethoxylate (15/4 EO/OH), 0.05 M ammonium sulfate, 0.05 M Bis-Tris, pH 6.0