6W3H
Brain delivery of therapeutic proteins using an Fc fragment blood-brain barrier transport vehicle in mice and monkeys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-20 |
Detector | DECTRIS PILATUS3 100K-M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 64 |
Unit cell lengths | 126.370, 126.370, 113.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 55.300 - 3.380 |
R-factor | 0.2415 |
Rwork | 0.238 |
R-free | 0.31820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s7g |
RMSD bond length | 0.012 |
RMSD bond angle | 1.770 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.300 | 3.440 |
High resolution limit [Å] | 3.380 | 3.380 |
Number of reflections | 14541 | 713 |
<I/σ(I)> | 7.2 | |
Completeness [%] | 99.7 | |
Redundancy | 9.5 | |
CC(1/2) | 0.930 | 0.560 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297.15 | 20-25% PEG3350, 0.1M Bis-Tris pH6.5, and 0.2M LiSO4 |