6W0M

Human 8-oxoguanine glycosylase crosslinked with oxoG lesion containing DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 65 2 2
Unit cell lengths	91.678, 91.678, 212.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.840 - 2.370
R-factor	0.2
Rwork	0.197
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ebm
RMSD bond length	0.009
RMSD bond angle	1.448
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.840	2.500
High resolution limit [Å]	2.370	2.370
Rmerge	0.086	0.746
Number of reflections	22361	3154
<I/σ(I)>	35.2
Completeness [%]	99.8
Redundancy	20.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM sodium cacodylate, pH 6.5, 200 mM MgCl2, and 18 % polyethylene glycol 8000.