6VZR

Engineered TTLL6 bound to the initiation analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97741
Spacegroup name	P 1 21 1
Unit cell lengths	75.099, 108.813, 171.618
Unit cell angles	90.00, 90.03, 90.00

Refinement procedure

Resolution	45.950 - 2.600
R-factor	0.231
Rwork	0.230
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ylr
RMSD bond length	0.002
RMSD bond angle	0.512
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.950	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.042	0.312
Rmeas	0.059	0.441
Rpim	0.042	0.312
Number of reflections	169504	16567
<I/σ(I)>	10.45	2.3
Completeness [%]	99.7	99.07
Redundancy	2	2
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	290	100 mM Sodium Citrate, 200 mM MgCl2, 8-12% Peg 20000