6VZL

Crystal structure of human PPARgamma ligand binding domain in complex with GW1929

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	92.849, 62.131, 119.116
Unit cell angles	90.00, 102.17, 90.00

Refinement procedure

Resolution	58.220 - 2.070
R-factor	0.253
Rwork	0.251
R-free	0.28860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1prg
RMSD bond length	0.009
RMSD bond angle	1.004
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.220	2.144
High resolution limit [Å]	2.070	2.070
Rmerge	0.051	1.374
Rmeas	0.056
Rpim	0.022
Number of reflections	40606	3920
<I/σ(I)>	13.59
Completeness [%]	98.8	97.88
Redundancy	6.4	6.4
CC(1/2)	0.999	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.1M MOPS, pH 7.4, 0.8M SODIUM CITRATE