6VVU
Anti-Tryptase fab E104.v1 bound to tryptase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2015-01-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.573, 168.811, 114.652 |
| Unit cell angles | 90.00, 109.97, 90.00 |
Refinement procedure
| Resolution | 90.830 - 3.000 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fvd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.830 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.860 | |
| Rpim | 0.582 | |
| Number of reflections | 62323 | 6175 |
| <I/σ(I)> | 11.3 | 1.37 |
| Completeness [%] | 97.8 | 97.8 |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.415 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 292 | 0.1 M Tris pH 8.5, 0.2 M CaCl2, 20% PEG 4000 and 4% pentaerythritol ethoxylate |
