6VTA

Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with amikacin and acetyl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	LIQUID ANODE
Source details	BRUKER METALJET
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-30
Detector	Bruker PHOTON II
Wavelength(s)	1.34
Spacegroup name	C 1 2 1
Unit cell lengths	93.367, 58.928, 83.203
Unit cell angles	90.00, 115.95, 90.00

Refinement procedure

Resolution	27.470 - 1.420
R-factor	0.1421
Rwork	0.140
R-free	0.18230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vr2
RMSD bond length	0.005
RMSD bond angle	0.940
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.470	1.471
High resolution limit [Å]	1.420	1.420
Number of reflections	76642	7629
<I/σ(I)>	21.92	2.42
Completeness [%]	99.4
Redundancy	2
CC(1/2)	0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277.15	Na3Cit, NaCl, TRIS, amikacin, acetyl CoA