6VMJ

Crystal structure of human Complement Factor D with anti-Factor D Fab 20D12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00003
Spacegroup name	P 41 21 2
Unit cell lengths	180.843, 180.843, 304.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.240 - 2.950
R-factor	0.218
Rwork	0.217
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d9r
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.060
High resolution limit [Å]	2.950	6.350	2.950
Rmerge	0.135	0.042	0.633
Total number of observations	767163
Number of reflections	106295	11214	10462
<I/σ(I)>	7
Completeness [%]	99.9	99.9	99.8
Redundancy	7.2	7.9	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 M sodium cacodylate pH 6.5, 1 M sodium citrate