6VIF
Human LRH-1 ligand-binding domain bound to agonist cpd 15 and fragment of coregulator TIF-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 46.134, 46.134, 223.436 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.640 - 2.260 |
| R-factor | 0.2423 |
| Rwork | 0.240 |
| R-free | 0.28360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6oqy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.508 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmeas | 0.217 | 0.903 |
| Rpim | 0.050 | |
| Number of reflections | 12218 | 2148 |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.7 | |
| Redundancy | 16.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | Na acetate (pH 4.6), PEG 4000, glycerol |
