6VGC
Crystal Structures of FLAP bound to DG-031
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.911, 101.488, 111.864 |
| Unit cell angles | 90.00, 99.83, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.370 |
| R-factor | 0.2554 |
| Rwork | 0.254 |
| R-free | 0.28480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.817 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.490 | 2.500 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 54437 | 3982 |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 100 mM MES pH 6.5, 26% PEG 350 MME, 200 mM Calcium Chloride, 1 mM DG-031 |
