6VG6

Crystal structure of DPO4 with 8-oxoadenine (oxoA) and dGTP*

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	120
Detector technology	PIXEL
Collection date	2019-04-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	52.975, 97.761, 101.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.150 - 3.080
Rwork	0.254
R-free	0.30210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
RMSD bond length	0.004
RMSD bond angle	0.776
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.140
High resolution limit [Å]	3.080	3.080
Rpim	0.059	0.461
Number of reflections	19085	1027
<I/σ(I)>	10.2
Completeness [%]	98.6
Redundancy	3.3
CC(1/2)		0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	16% PEG3350 50 mM Tris 100 mM Magnesium acetate