6VC7

Structure of the F349A mutant of the periplasmic domain of YejM from Salmonella typhimurium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	80
Detector technology	CMOS
Collection date	2018-03-14
Detector	RDI CMOS_8M
Wavelength(s)	1.001
Spacegroup name	P 21 21 21
Unit cell lengths	121.612, 124.899, 183.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.560 - 2.050
R-factor	0.1866
Rwork	0.184
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vat
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.670	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	150136	8464
<I/σ(I)>	13.8
Completeness [%]	85.9	48.97
Redundancy	6.4
CC(1/2)	0.997	0.184

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	291	0.1 M HEPES pH 6.8, 5% PEG3350